| EST name | EST sequence |
| gi|75459553|gb|DT767876.1|DT767876 | CCAGTACCCTAAACTCTCTTCTTCTCAAAAAATTAACAATGGCTTCAATGACTACTGCTTTCACTTCTCATCTTTCTTCC AAAATCAAAACCCCTTTCTTAGATAACCAATCATCTTTCCATGTCACTCCATCTCGCATCCAACTCACTCGTTCATCTTC CCCAAACACTCACATCACCATGTCTTCGTCATCATCATCATCATCTTCATCTTATGATCTATCTTCCTTCAAATTTGAAC CAATTAAGGAATCCATTGTATCACGTGAGATGACTCGCAGATACATGATGGATATGATTACATATGCTGACACTGATGTT GTTGTTGTTGGAGCAGGTTCAGCAGGTCTTTCTTGTGCTTATGAAATGAGCAAAAACCCTAACATCAACATCGCTATTAT CGAACAATCTGTCAGTCCTGGTGGTGGTGCTTGGTTAGGCGGTCAGCTTTTTTCTGCCATGGTTGTTCGCAAACCTGCTC ACATCTTCCTTGATGAACTCGGTGTTGAATACGATGAACAAGACACCTATGTTGTTATCAAGCACGCTGCCTTGTTTACA TCCACAATCATGTCTAAGCTTTTGGCTAAACCAAATGTGAAACTTTTTAATGCTGTTGCTGCTGAGGATTTGATCGTGAA AGAAGGTAAAGTTGCTGGGGTTGTCACAAATTGGGCTTTGGTTTCTATGAATCATGATACACAATCATGTATGGATCCAA ATGTAATGGAAGCTAAGATTGTTGTCAGTTCTTGTGGCCATGATGGTCCTTTTGGTGCTACTGGTGTGAAGAGGCTGAGA GCATTGGAATGATTGATTCTGTTCCTGGTATGAAAGCTCTTGATATGATACTGCTGAGGATGCTATTGTCAGA |
Most similar to sp|O23787|THI4_CITSI
>BLASTX 2.2.20 [Feb-08-2009]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
>sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplastic
OS=Citrus sinensis GN=THI1 PE=2 SV=1
Length = 356
Score = 356 bits (914), Expect(2) = e-100
Identities = 190/257 (73%), Positives = 202/257 (78%), Gaps = 2/257 (0%)
Frame = +3
Query: 39 MASM-TTAFTSHLSSKIKTPFLDNQSSFHVTPSRIQLTRXXXXXXXXXXXXXXXXXXXXX 215
MA+M +TAF +SS F SSFH P L R
Sbjct: 1 MAAMASTAFAPSVSSTTNKLF---DSSFHGAPMSPSLLRLQPIKSSRPNNLSISASASPP 57
Query: 216 XXXX-FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINI 392
FKF+PIKESIVSREMTRRYM DMITYADTD GAGSAGLSCAYE+SKNPNI I
Sbjct: 58 YDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQI 117
Query: 393 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIM 572
AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELG++YDEQD YVVIKHAALFTSTIM
Sbjct: 118 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIM 177
Query: 573 SKLLAKPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSS 752
SKLLA+PNVKLFNAVAAEDLIVK G+V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSS
Sbjct: 178 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 237
Query: 753 CGHDGPFGATGVKRLRA 803
CGHDGPFGATGVKRL++
Sbjct: 238 CGHDGPFGATGVKRLKS 254
Score = 32.3 bits (72), Expect(2) = e-100
Identities = 14/17 (82%), Positives = 16/17 (94%)
Frame = +2
Query: 797 ESIGMIDSVPGMKALDM 847
+SIGMI+ VPGMKALDM
Sbjct: 253 KSIGMIEEVPGMKALDM 269
>sp|Q38709|THI4_ALNGL Thiazole biosynthetic enzyme, chloroplastic
OS=Alnus glutinosa GN=THI4 PE=2 SV=1
Length = 352
Score = 340 bits (872), Expect(2) = 3e-96
Identities = 167/192 (86%), Positives = 179/192 (93%)
Frame = +3
Query: 228 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINIAIIEQ 407
F F+PIKESIVSREMTRRYMMDMITYADTD GAGS+GL C YE+SKNP++ +AIIEQ
Sbjct: 60 FTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIEQ 118
Query: 408 SVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLLA 587
SVSPGGGAWLGGQLFS MVVRKPAH+FLDELG+EYDEQD YVVIKHAALFTSTIMSKLLA
Sbjct: 119 SVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLLA 178
Query: 588 KPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 767
+PNVKLFNAVAAEDLIVK G+V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 179 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 238
Query: 768 PFGATGVKRLRA 803
PFGATGVK LR+
Sbjct: 239 PFGATGVKSLRS 250
Score = 33.9 bits (76), Expect(2) = 3e-96
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +2
Query: 800 SIGMIDSVPGMKALDM 847
SIGMID+VPGMKALDM
Sbjct: 250 SIGMIDTVPGMKALDM 265
>sp|Q38814|THI4_ARATH Thiazole biosynthetic enzyme, chloroplastic
OS=Arabidopsis thaliana GN=THI1 PE=1 SV=1
Length = 349
Score = 337 bits (865), Expect(2) = 2e-95
Identities = 164/192 (85%), Positives = 178/192 (92%)
Frame = +3
Query: 228 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINIAIIEQ 407
F F+PIKESIVSREMTRRYM DMITYA+TD GAGSAGLS AYE+SKNPN+ +AIIEQ
Sbjct: 56 FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 115
Query: 408 SVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLLA 587
SVSPGGGAWLGGQLFSAM+VRKPAH+FLDE+GV YDEQDTYVV+KHAALFTSTIMSKLLA
Sbjct: 116 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 175
Query: 588 KPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 767
+PNVKLFNAVAAEDLIVK +V GVVTNWALV+ NH TQSCMDPNVMEAKIVVSSCGHDG
Sbjct: 176 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 235
Query: 768 PFGATGVKRLRA 803
PFGATGVKRL++
Sbjct: 236 PFGATGVKRLKS 247
Score = 33.5 bits (75), Expect(2) = 2e-95
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +2
Query: 797 ESIGMIDSVPGMKALDM 847
+SIGMID VPGMKALDM
Sbjct: 246 KSIGMIDHVPGMKALDM 262
>sp|Q41738|THI41_MAIZE Thiazole biosynthetic enzyme 1-1,
chloroplastic OS=Zea mays GN=THI1-1 PE=2 SV=1
Length = 354
Score = 335 bits (859), Expect(2) = 8e-94
Identities = 163/191 (85%), Positives = 177/191 (92%)
Frame = +3
Query: 228 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINIAIIEQ 407
F+F PIKESIVSREMTRRYM DMITYADTD GAGSAGLSCAYE+SK+P ++IAI+EQ
Sbjct: 62 FRFSPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPAVSIAIVEQ 121
Query: 408 SVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLLA 587
SVSPGGGAWLGGQLFSAMVVRKPAH+FLDELGV YDE + YVVIKHAALFTST+MS LLA
Sbjct: 122 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDELGVAYDEAEDYVVIKHAALFTSTVMSLLLA 181
Query: 588 KPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 767
+PNVKLFNAVA EDLIV+ G+V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 182 RPNVKLFNAVAVEDLIVRGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 241
Query: 768 PFGATGVKRLR 800
PFGATGVKRL+
Sbjct: 242 PFGATGVKRLQ 252
Score = 30.8 bits (68), Expect(2) = 8e-94
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +2
Query: 797 ESIGMIDSVPGMKALDM 847
+ IGMI +VPGMKALDM
Sbjct: 252 QDIGMISAVPGMKALDM 268
>sp|Q41739|THI42_MAIZE Thiazole biosynthetic enzyme 1-2,
chloroplastic OS=Zea mays GN=THI1-2 PE=2 SV=1
Length = 354
Score = 334 bits (856), Expect(2) = 2e-93
Identities = 159/191 (83%), Positives = 178/191 (93%)
Frame = +3
Query: 228 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEMSKNPNINIAIIEQ 407
F+F PIKESIVSREMTRRYM DMIT+ADTD GAGSAGLSCAYE+SK+P +++AI+EQ
Sbjct: 59 FRFSPIKESIVSREMTRRYMTDMITHADTDVVIVGAGSAGLSCAYELSKDPTVSVAIVEQ 118
Query: 408 SVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLLA 587
SVSPGGGAWLGGQLFSAMVVR+PAH+FLDELGV YDE + YVV+KHAALFTST+MS+LLA
Sbjct: 119 SVSPGGGAWLGGQLFSAMVVRRPAHLFLDELGVGYDEAEDYVVVKHAALFTSTVMSRLLA 178
Query: 588 KPNVKLFNAVAAEDLIVKEGKVAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 767
+PNVKLFNAVA EDLIV+ G+V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 179 RPNVKLFNAVAVEDLIVRRGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 238
Query: 768 PFGATGVKRLR 800
PFGATGVKRL+
Sbjct: 239 PFGATGVKRLQ 249
Score = 30.8 bits (68), Expect(2) = 2e-93
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +2
Query: 797 ESIGMIDSVPGMKALDM 847
+ IGMI +VPGMKALDM
Sbjct: 249 QDIGMISAVPGMKALDM 265
>sp|P40998|THI2_SCHPO Thiazole biosynthetic enzyme, mitochondrial
OS=Schizosaccharomyces pombe (strain ATCC 38366 / 972)
GN=thi2 PE=2 SV=2
Length = 328
Score = 259 bits (661), Expect = 2e-68
Identities = 130/196 (66%), Positives = 156/196 (79%), Gaps = 6/196 (3%)
Frame = +3
Query: 228 FKFEPIKESIVSREMTRRYMMDMITYADTDXXXXGAGSAGLSCAYEM-SKNPNINIAIIE 404
F F PI+ES VSR MTRRY D+ YA++D GAGSAGL+ AY + ++ P++ IAIIE
Sbjct: 49 FSFAPIRESTVSRAMTRRYFSDLDKYAESDIVIVGAGSAGLTAAYYIGTRRPDLKIAIIE 108
Query: 405 QSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGVEYDEQDTYVVIKHAALFTSTIMSKLL 584
SV+PGGGAWLGGQLFSAMVVRKPA +FL+E+GV Y+++ YVV+KHAALFTST+M++ L
Sbjct: 109 ASVAPGGGAWLGGQLFSAMVVRKPADLFLNEIGVPYEDEGDYVVVKHAALFTSTVMARTL 168
Query: 585 AKPNVKLFNAVAAEDLIVKEGK-----VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVS 749
A PNVKLFNA A EDLIVKEGK +AGVVTNW LVS+NH QSCMDPN + A +VVS
Sbjct: 169 ALPNVKLFNATAVEDLIVKEGKDGKQRIAGVVTNWTLVSLNHGLQSCMDPNTINAHLVVS 228
Query: 750 SCGHDGPFGATGVKRL 797
+ GHDGPFGA VKRL
Sbjct: 229 ATGHDGPFGAFCVKRL 244